Search results for " display techniques"
showing 6 items of 6 documents
Characterization of a Novel Conformational GII.4 Norovirus Epitope: Implications for Norovirus-Host Interactions
2016
ABSTRACT Human noroviruses (NoVs) are the main etiological agents of acute gastroenteritis worldwide. While NoVs are highly diverse (more than 30 genotypes have been detected in humans), during the last 40 years most outbreaks and epidemics have been caused by GII.4 genotype strains, raising questions about their persistence in the population. Among other potential explanations, immune evasion is considered to be a main driver of their success. In order to study antibody recognition and evasion in detail, we analyzed a conformational epitope recognized by a monoclonal antibody (3C3G3) by phage display, site-directed mutagenesis, and surface plasmon resonance. Our results show that the predi…
Precise mapping of the Goodpasture epitope(s) using phage display, site-directed mutagenesis, and surface plasmon resonance.
2013
Goodpasture disease is an autoimmune disorder mediated by circulating autoantibodies against the noncollagenous-1 (NC1) domain of the alpha 3 chain of type IV collagen (alpha 3(IV)NC1). The structure of Goodpasture epitope(s) has been previously mapped into two main binding regions (E-A and E-B) of the alpha 3(IV)NC1 domain using a residue mutation approach on the highly related alpha 1(IV)NC1 domain. Here we combined phage display and surface plasmon resonance technology to more precisely localize the pathogenic binding sites. Peptides mimicking the Goodpasture epitope(s) were used to identify residues involved in autoantibody binding and found involvement of eight residues previously unre…
New technology for archaeological museums: three case studies on the Mediterranean coast
2013
The archaeology/technology combination has long been accepted with the second being seen to be at the service of the study and conservation of the first. Much less immediate and recent is the combination between the archaeological museum and technology for communication. Certainly, becauseof its ability to express dynamic and immediate information, new technology, if well integrated in the exhibition and well calibrated with the presence of original objects, can be a very effective support to the public’s appreciation. The paper aims to provide some examples that seem to adequately describe good integration practices between the authentic and the mediated, between the original and the virtu…
The Museum of London, a City Museum as Narrative Exhibition
2014
From temples of sacred objects to repositories of colonial trophies and from monuments of civility to spaces for self-expression, the shape and the appeal of museums through history has been fashioned to hold particular collections of objects. Moreover the museums have been also designed to adapt to particular display techniques appropriate for a particular collection. In the last century, a period of structural reinvention in the design and shaping of the exhibitions, the museum making is challenging, creative, and complex. The ‘new museum making’ includes emotive exhibitions within communicative buildings in order to create ‘narrative experiences’ which integrate objects, spaces, and audi…
In vivo phage display: identification of organ-specific peptides using deep sequencing and differential profiling across tissues.
2021
Abstract In vivo phage display is widely used for identification of organ- or disease-specific homing peptides. However, the current in vivo phage biopanning approaches fail to assess biodistribution of specific peptide phages across tissues during the screen, thus necessitating laborious and time-consuming post-screening validation studies on individual peptide phages. Here, we adopted bioinformatics tools used for RNA sequencing for analysis of high-throughput sequencing (HTS) data to estimate the representation of individual peptides during biopanning in vivo. The data from in vivo phage screen were analyzed using differential binding—relative representation of each peptide in the target…
Museum and Exhibition Design in the Digital Age
2013
From temples of sacred objects to repositories of colonial trophies and from monuments of civility to spaces for self-expression, the shape and the appeal of museums through history has been fashioned to hold particular collections of objects. Moreover the museums have been also designed to adapt to particular display techniques appropriate for a particular collection. In the last century, a period of structural reinvention in the design and shaping of the exhibitions, the museum making is challenging, creative, and complex. The ‘new museum making’ includes emotive exhibitions within communicative buildings in order to create ‘narrative experiences’ which integrate objects, spaces, and audi…